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Selection of Entomopathogenic Nematodes to Control Nymphs of Haplaxius crudus (Van Duzee) (Hemiptera: Cixiidae)

Received: 29 January 2019     Accepted: 20 March 2019     Published: 15 May 2019
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Abstract

Haplaxius crudus transmits the pathogen that causes Lethal wilt (ML for its name in Spanish), one of the main diseases that affects oil palms in Colombia. In its nymphal stage it feeds from grasses present at the plantations, and adults feed on oil palm leaves. With the objective of controlling the nymphal stage of H. crudus, the effectiveness of the following entomopathogenic nematodes was assessed: Steinernema colombiense, S. websteri, Steinernema sp. 1, Steinernema sp. 2, Heterorhabditis bacteriophora, Heterorhabditis sp. (Gua 31), Heterorhabditis sp. (Gua 236), Heterorhabditis sp. (CPHsp1301) and Heterorhabditis sp. (CPHsp1302). Pathogenicity was assessed under laboratory conditions using Petri dishes with roots of Paspalum virgatum. Virulence was assessed using mesh houses with PVC tubes and plastic trays with P. virgatum. Once the most virulent nematode was selected, three dosage levels were assessed under simulated field conditions, in order to select the most effective dosage. All assessed nematode species were pathogenic to nymphs of H. crudus, and nymph stage IV was the most susceptible stage, with death rates of over 80%. Regarding virulence, there were statistically significant differences among treatments (P≤0.05), producing nematode death rates between 28.3 and 88.2%. Heterorhabditis sp. (CPHsp1301), obtained from the soil of palm plantations, was selected because it caused a mortality rate of 78.4% with at a dosage of 1300 IJ/cm2 in the sprayed area. The results are promising and further research should be performed under commercial oil palm plantation conditions.

Published in American Journal of Entomology (Volume 3, Issue 1)
DOI 10.11648/j.aje.20190301.14
Page(s) 24-29
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Oil Palm, Biological Control, Steinernema, Heterorhabditis

References
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  • APA Style

    Miriam Rosero Guerrero, Alex Enrique Bustillo Pardey. (2019). Selection of Entomopathogenic Nematodes to Control Nymphs of Haplaxius crudus (Van Duzee) (Hemiptera: Cixiidae). American Journal of Entomology, 3(1), 24-29. https://doi.org/10.11648/j.aje.20190301.14

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    ACS Style

    Miriam Rosero Guerrero; Alex Enrique Bustillo Pardey. Selection of Entomopathogenic Nematodes to Control Nymphs of Haplaxius crudus (Van Duzee) (Hemiptera: Cixiidae). Am. J. Entomol. 2019, 3(1), 24-29. doi: 10.11648/j.aje.20190301.14

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    AMA Style

    Miriam Rosero Guerrero, Alex Enrique Bustillo Pardey. Selection of Entomopathogenic Nematodes to Control Nymphs of Haplaxius crudus (Van Duzee) (Hemiptera: Cixiidae). Am J Entomol. 2019;3(1):24-29. doi: 10.11648/j.aje.20190301.14

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  • @article{10.11648/j.aje.20190301.14,
      author = {Miriam Rosero Guerrero and Alex Enrique Bustillo Pardey},
      title = {Selection of Entomopathogenic Nematodes to Control Nymphs of Haplaxius crudus (Van Duzee) (Hemiptera: Cixiidae)},
      journal = {American Journal of Entomology},
      volume = {3},
      number = {1},
      pages = {24-29},
      doi = {10.11648/j.aje.20190301.14},
      url = {https://doi.org/10.11648/j.aje.20190301.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aje.20190301.14},
      abstract = {Haplaxius crudus transmits the pathogen that causes Lethal wilt (ML for its name in Spanish), one of the main diseases that affects oil palms in Colombia. In its nymphal stage it feeds from grasses present at the plantations, and adults feed on oil palm leaves. With the objective of controlling the nymphal stage of H. crudus, the effectiveness of the following entomopathogenic nematodes was assessed: Steinernema colombiense, S. websteri, Steinernema sp. 1, Steinernema sp. 2, Heterorhabditis bacteriophora, Heterorhabditis sp. (Gua 31), Heterorhabditis sp. (Gua 236), Heterorhabditis sp. (CPHsp1301) and Heterorhabditis sp. (CPHsp1302). Pathogenicity was assessed under laboratory conditions using Petri dishes with roots of Paspalum virgatum. Virulence was assessed using mesh houses with PVC tubes and plastic trays with P. virgatum. Once the most virulent nematode was selected, three dosage levels were assessed under simulated field conditions, in order to select the most effective dosage. All assessed nematode species were pathogenic to nymphs of H. crudus, and nymph stage IV was the most susceptible stage, with death rates of over 80%. Regarding virulence, there were statistically significant differences among treatments (P≤0.05), producing nematode death rates between 28.3 and 88.2%. Heterorhabditis sp. (CPHsp1301), obtained from the soil of palm plantations, was selected because it caused a mortality rate of 78.4% with at a dosage of 1300 IJ/cm2 in the sprayed area. The results are promising and further research should be performed under commercial oil palm plantation conditions.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Selection of Entomopathogenic Nematodes to Control Nymphs of Haplaxius crudus (Van Duzee) (Hemiptera: Cixiidae)
    AU  - Miriam Rosero Guerrero
    AU  - Alex Enrique Bustillo Pardey
    Y1  - 2019/05/15
    PY  - 2019
    N1  - https://doi.org/10.11648/j.aje.20190301.14
    DO  - 10.11648/j.aje.20190301.14
    T2  - American Journal of Entomology
    JF  - American Journal of Entomology
    JO  - American Journal of Entomology
    SP  - 24
    EP  - 29
    PB  - Science Publishing Group
    SN  - 2640-0537
    UR  - https://doi.org/10.11648/j.aje.20190301.14
    AB  - Haplaxius crudus transmits the pathogen that causes Lethal wilt (ML for its name in Spanish), one of the main diseases that affects oil palms in Colombia. In its nymphal stage it feeds from grasses present at the plantations, and adults feed on oil palm leaves. With the objective of controlling the nymphal stage of H. crudus, the effectiveness of the following entomopathogenic nematodes was assessed: Steinernema colombiense, S. websteri, Steinernema sp. 1, Steinernema sp. 2, Heterorhabditis bacteriophora, Heterorhabditis sp. (Gua 31), Heterorhabditis sp. (Gua 236), Heterorhabditis sp. (CPHsp1301) and Heterorhabditis sp. (CPHsp1302). Pathogenicity was assessed under laboratory conditions using Petri dishes with roots of Paspalum virgatum. Virulence was assessed using mesh houses with PVC tubes and plastic trays with P. virgatum. Once the most virulent nematode was selected, three dosage levels were assessed under simulated field conditions, in order to select the most effective dosage. All assessed nematode species were pathogenic to nymphs of H. crudus, and nymph stage IV was the most susceptible stage, with death rates of over 80%. Regarding virulence, there were statistically significant differences among treatments (P≤0.05), producing nematode death rates between 28.3 and 88.2%. Heterorhabditis sp. (CPHsp1301), obtained from the soil of palm plantations, was selected because it caused a mortality rate of 78.4% with at a dosage of 1300 IJ/cm2 in the sprayed area. The results are promising and further research should be performed under commercial oil palm plantation conditions.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Colombian Oil Palm Research Center, Paratebueno, Colombia

  • Colombian Oil Palm Research Center, Bogotá, Colombia

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